POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION
نویسندگان
چکیده
منابع مشابه
Polynucleotide ligase in bacteriophage T4D recombination.
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) i...
متن کاملReplication and recombination in ligase-deficient rII bacteriophage T4D.
Deoxyribonucleic acid replication and genetic recombination were investigated after infection of Escherichia coli with ligase-deficient rII bacteriophage T4D. The major observations are: (i) deoxyribonucleic acid synthesis is discontinuous, (ii) the discontinuities are more slowly repaired than in wild-type infection, (iii) host ligase is required for viability, and (iv) genetic recombination i...
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Mutations in the Escherichia coli genes recK, recL and (probably) uvrE and polA increase special (glucosylation-dependent), but not general recombination in bactriophage T4D.
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The mechanism of action of T4-induced polynu-cleotide kinase on short single-stranded deoxyoligonucleotideshas been investigated.It was found that the phosphorylationreaction catalyzed by T, polynucleotide kinase could be re-versed. Thus, a 5 ’-3*P-labeleddeoxyoligonucleotide and ADPon incubation with the enzyme formed [Y-~~PJATP.In addi-tion some radioac...
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Various properties of the bacteriophage structural dihydrofolate reductase (DFR) have been examined to determine its function during phage infection. It has been found that a binding site for reduced nicotinamide adenine dinucleotide phosphate (NADPH), most likely on the DFR present in the phage tail plate, is required for phage viability. Attachment of adenosine diphosphoribose, an analogue of...
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ژورنال
عنوان ژورنال: Genetics
سال: 1972
ISSN: 1943-2631
DOI: 10.1093/genetics/72.2.187